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pupG [2019-07-04 13:40:07]
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pupG [2019-07-04 13:40:07]

purine nucleoside phosphorylase
locus
BSU23490
pI
4.86
mw
28.98 kDa
protein length
271 aa Sequence Blast
gene length
816 bp Sequence Blast
function
purine salvage and interconversion
product
purine nucleoside phosphorylase
essential
no
ec
2.4.2.1
synonyms
punA,pnp,yqkO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.3|Purine salvage and interconversion]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    2,446,418 → 2,447,233

    The protein

    Catalyzed reaction/ biological activity

  • Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate (according to Swiss-Prot) RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate (according to Swiss-Prot)

    • Protein family

  • PNP/MTAP phosphorylase family (according to Swiss-Prot) polyribonucleotide nucleotidyltransferase family (according to Swiss-Prot)

    • Modification

  • phosphorylation on Ser-28 [Pubmed|17218307]

    • Structure

  • [PDB|3LA8] (from Streptococcus mutans, 57% identity)

    • [SW|Localization]

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|9D07E43BF1FFB13CCEAC2B7AAA4EBDFB1079DD74|drm]-[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]
    description
    [Pubmed|10537218]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10537218], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) [Pubmed|10537218]
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    Biological materials

    Mutant

  • BKE23490 (Δ[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGG
  • BKK23490 (Δ[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGG

    • References

  • 10537218,22900538,17218307